Averrhoa bilimbi: A Prospective Source of BioactiveCompounds against Antimicrobial and Cytotoxic Activities.
Category:- Journal; Year:- 2021
Discipline:- Chemistry Discipline
School:- Science, Engineering & Technology School
Abstract
Averrhoa bilimbi fruit has been studied to find out a possible source
of bioactive compounds against antimicrobial and cytotoxic activity. To achieve
this goal, total phenolic content and total flavonoid content, antioxidant,
antimicrobial and cytotoxic activities of five different fractionates such as
methanolic crude, petroleum ether, chloroform, ethyl acetate and water fraction
of A. bilimbi were investigated by UV-vis spectrophotometry, disc diffusion and
brine shrimp lethality test methods simultaneously. Other phytochemicals viz.
carbohydrate, tannins, polyphenols, glycosides, etc. were also screened out by
conventional test methods concurrently. Total phenolic contents and total
flavonoid contents were found to be ranging from 3.807-262.129 mg GAE g–1 and
67.848-1159.061 mg CE g–1, respectively, in all the analyzed fractions. DPPH
free radical scavenging activity revealed distinctive IC50 value of 5.63 and
10.26 μg mL–1 in the chloroform and ethyl acetate extractives. Fractionates of
petroleum ether and methanolic crude also presented strong cytotoxic potential
with the LD50 values of 8.89 and 20.3 μg mL–1 in the brine shrimp lethality
bioassay test. Petroleum ether and ethyl acetate fractions exhibited good
antimicrobial activity with the zone of inhibition against Bacillus cereus (11
mm and 14 mm) and Escherichia coli (7 mm and 9 mm). Finally, it has been
recommended that A. bilmbi fruit might be an effective source of bioactive
compounds against antimicrobial and cytotoxic activity as well as antioxidant
with potent radical scavenging activity.