Apoptosis on non-small lung cancer cells and DPPH radical scavenging activity of the crude ethyl acetate extract of a soil bacterial species FEAI-1

Author:- Most. Farida Khatun, Md. Uzzal Haque, Abu Syed Md Anisuzzaman, Md. Anwar Ul Islam
Category:- Journal; Year:- 2021
Discipline:- Pharmacy Discipline
School:- Life Science School


The aim of this research is to evaluate the in vitro apoptosis and free radical scavenging activities of the crude extract of a soil bacterial species FEAI-1. Apoptosis activity was investigated using annexin V-phycoerythrin staining on human non-small-cell lung carcinoma cell line (H1299), and an antioxidant study of the crude ethyl acetate extract was carried out by the diphenylpicrylhydrazyl (DPPH) assay. The crude ethyl acetate extract of the bacterial species FEAI-1 induced apoptosis on H1299 cell lines in a concentration-dependent manner. After a 96-hours treatment period, flow cytometry showed that the extract induced total apoptosis by 10.25%, 13.48%, 20.42%, 68.10%, and 68.80% at concentrations of 2.5, 5, 10, 20, and 40 μg/ml, respectively. The crude extracts which elicited antioxidant activity with the inhibition of DPPH radicals were 24.97% ± 0.288%, 26.29% ± 0.085%, 29.06% ± 0.123%, 33.07% ± 0.01%, 38.45% ± 0.03%, 45.33% ± 0.02%, and 53.21% ± 0.115% at concentrations (μg/ml) of 10, 20, 40, 80, 160, 320, and 640, respectively. The inhibition concentration 50 value of the extract was 510 μg/ml. These results suggest that the crude extract of the bacterial sp. FEAI-1 could be a useful natural source having therapeutic potential against cancer and diseases caused by oxidative stress. Furthermore, in vivo studies and compound isolation pave the way for its biomedical applications.

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