Green Synthesis of Silver Nanoparticles using Piper chaba Stem Extract: Investigation of their Antibacterial and Antioxidant Properties

Green synthesis of silver nanoparticles (AgNPs) was conducted using the stem extract of Piper Chaba, which is a plant abundantly growing in South and Southeast Asia. The synthesis was carried out at different reaction conditions, i.e., reaction temperature, concentrations of the extract and silver nitrate, reaction time, and pH. The synthesized AgNPs were characterized by visual observation, ultraviolet-visible (UV-vis) spectroscopy, dynamic light scattering (DLS), scanning electron microscopy (SEM), transmission electron microscopy (TEM), x-ray diffraction (XRD), energy dispersive x-ray (EDX), and Fourier transform infrared (FTIR) spectroscopy. The characterization results revealed that AgNPs were uniformly dispersed and exhibited a moderate size distribution. They were mostly spherical crystals with face-centered cubic structures and an average size of 19 nm. The FTIR spectroscopy and DLS analysis indicated that the phytochemicals capping the surface of AgNPs stabilize the dispersion through anionic repulsion. The synthesized AgNPs showed good antibacterial activity and significant antioxidant activity.

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