EFFECT OF 2,4-D ON CALLUS INDUCTION AND PLANT REGENERATION IN THREE INDEGENOUS AROMATIC RICE CULTIVARS OF BANGLADESH

The present investigation was carried out to find out the most suitable concentrations of 2, 4- D and the potentiality of selected aromatic local rice cultivars for maximum callus induction and plant regeneration. Matured dehusked seeds of three local aromatic rice cultivars viz Raniselute, Kachda and Chiniatap were cultured on MS medium supplemented with different concentrations of 2,4-D at 0.5, 1.0, 1.5, 2.0, 2.5, 3.0 mgL^-1. MS media supplemented with 2, 4-D at 1.0 mg L^-1 performed better for callus induction in Chiniatap (88%) and in Raniselute (76%) at 2.0 mg L^-12,4-D, Kachda performed better (76%) than other concentration. The cultivar Kachda showed comparatively better performance in morphogenesis (90.05%) than Raniselute (84.89 %) and Chiniatap (71.46 %). MS media supplemented with 2,4-D at 2.0 mgL^-1 performed the best for morphogenesis (90.9%) in Raniselute and ( 90%) in Chiniatap. The cultivar Kachda showed comparatively better performance (23.67%) in plantlet regeneration than Raniselute (14.69%) and Chiniatap (7%). MS media supplemented with 2,4-D at 1.0 mgL^-1 performed the best in Raniselute (20.83%) and in Chiniatap (13.33%)  for plantlet regeneration. But in Kachda , it was best (31.58%) in 2.0mgL^-1. The rate of survivability of  Kachda (32%) was higher than Raniselute (9%) and Chiniatap (8%). In this respect, the cultivar Kachda and MS medium supplemented with 2, 4-D at 1.0 mgL^-1and 2.0 mgL^-1may be recommended for the further study of somaclonal variation and for genetic engineering studies.

• Supervision