S M Mahbubur Rahman
Professor
Email:
Satkhira road
Contact:
+880-41-720171 Extn 2222
Address:
Satkhira road, Khulna, Bangladesh
Ph.D. from Graduate School of Agriculture, Kobe University, Japan in 1997. Major in Molecular Genetics
MS from Graduate School of Agriculture, Kobe University, Japan in 1997. Major Plant cell and Tissue Culture
B. Sc. Agriculture (Hons) from, Shere Bangla Agricultural University, Bangladesh
Hydrocarbons such
as petrol, diesel, etc. contamination of land, and surface water is an extensive
worldwide environmental pollution issue from the reclamation and refining of
crude oil. Oil spills endanger public health, compromise drinking water,
devastate natural resources, and rattle the economy. Biodegradation of the
hydrocarbon occurs when microorganisms, especially bacteria, feed on oil hydrocarbons.
A wide range of potential microorganisms is necessary for a significant
reduction of the oil. Although most biosurfactants are considered secondary
metabolites, some may play crucial roles in the survival of
biosurfactant-producing microorganisms through expedited nutrient transport or microbe-host
interactions or by acting as biocide agents. Biosurfactant roles include
increasing the surface area and bioavailability of hydrophobic water-insoluble
substrates, heavy metal binding, bacterial pathogenesis, quorum sensing, and
biofilm formation. Bacteria produce a wide range of biosurfactants with a higher
rate of hydrocarbon biodegradability, and higher specific activity at extreme
temperatures, salinity, and pH levels that are considered environmentally
compatible with limited toxicity. Such kinds of natural microbial compounds are
biologically surface-active agents and possess many potential activities over
synthetic relatives. Previously microorganisms were considered to be harmful to
the petroleum industry, but now it is well known that microorganisms can be helpful
in terms of oil recovery also. Biosurfactants are characteristically
amphipathic bioactive compounds and contain hydrophobic and hydrophilic
portions. They actually act between fluids of different polarities (oil/water
and water/oil), thus, allowing access to hydrophobic substrates and thereby reducing
the surface tension, also enhancing the mobility, bioavailability, and
biodegradation of hydrocarbons. Bioremediation is an encouraging approach to the
hydrocarbon spills problem, as this employs microorganisms to degrade
hydrocarbons from the environment. The ability to degrade recalcitrant
compounds in petroleum has been reported for several bacterial genera isolated
from petroleum-contaminated soil having the potential for hydrocarbon
degradation.
My research interest is:
Microbial Biosurfactant for petroleum degradation
Current Research Project/Collaboration
1. Served as Subject Matter Officer, Department of Agricultural Extension, Ministry of Aviculture from 20/12/1989 to 30/09/1991
2. Served as Senior Scientific Officer, Bangladesh Sugarcane Research Institute, Ishurdi, Pabna, Bangladesh from 4/10/1998 to 24/04/1999.
3. Twenty-four Years of teaching experience at Biotechnology and Genetic Engineering Discipline, Khulna University, Bangladesh
No Data Available
Supervision
No Data Available
coming soon...
Farza Year 2022
1.
Rahagir
Salekeen, Fouzia Akhter, Md Emdadul Islam, S M Mahbubur Rahman, Md Morsaline
Billah, Kazi Mohammed Didarul Islam (2022). Citrus Fruits as Source of Novel
Bioactive Peptides: in-vitro and in-silico Analysis of Atheroprotective
Potentials. Journal of Food and Function, https://doi.org/10.1039/D2FO01129H.
Sakib Mahmud, Kazi Mohammed Didarul Islam, Md. Morsaline Billah, S. M. Mahbubur Rahman, Rana Biswas, Md. Emdadul Islam (2022). Evaluation of antioxidant and anticoagulation activity of Piper chaba stem. Pharmaceutical and Biomedical Research. 8(2):113-120. http://dx.doi.org/10
YEAR 2021
1.
Rahagir
Salekeen, Fouzia Akhter, Md Emdadul Islam, S M Mahbubur Rahman, Md Morsaline
Billah, Kazi Mohammed Didarul Islam (2022). Citrus Fruits as Source of Novel
Bioactive Peptides: in-vitro and in-silico Analysis of Atheroprotective
Potentials. Journal of Food and Function, https://doi.org/10.1039/D2FO01129H.
2.
Sakib Mahmud, Kazi Mohammed
Didarul Islam, Md. Morsaline
Billah, S. M. Mahbubur
Rahman, Rana Biswas, Md. Emdadul
Islam
(2022). Evaluation of antioxidant and anticoagulation activity of
Piper chaba stem. Pharmaceutical and
Biomedical Research. 8(2):113-120. http://dx.doi.org/10
YEAR 2021
1.
Rahagir
Salekeen , Joydip Barua, Punam Rani Shaha, Kazi Mohammed Didarul Islam, Md
Emdadul Islam, Md Morsaline Billah and S. M. Mahbubur Rahman, 2021.
Marine phycocompound screening reveals a potential source of novel
senotherapeutics, Journal of Biomolecular Structure & Dynamics, https://doi.org/10.1080/07391102.2021.1877822
P 1-15.
YEAR 2020
2.
K.
M. Hossain, B. Mazumder, S.M.M. Rahman and M.A. Hamid, 2020. Genetic diversity
analysis of lactic acid bacteria from yogurt samples of Bangladesh using
RAPD-PCR technique, Bangladesh Journal of Livestock Research, Vol. 27
(1&2): 55-63. https://doi.org/10.3329/bjlr.v27i1.55169
3.
K.
M. Hossain, A.K. Das, S.M.M. Rahman, S. Roy and M.A. Hamid, 2020. Biochemical
property analysis of native probiotic isolates from selective poultry,
Bangladesh Journal of Livestock Research, Vol. 27 (1&2): 39-54
https://doi.org/10.3329/bjlr.v27i1.55168
4.
Tauhidur
Rahman Nurunnabi, Sabiha Sarwar, Farah Sabrin, Farzana Alam, Lutfun Nahar,
Hossain Sohrab, Satyajit D. Sarker, S. M. Mahbubur Rahman and Morsaline Billah,
2020. Molecular identification and antimicrobial activity of endophytic fungi
isolated from Heritiera fomes (Buch. -Ham), a mangrove plant of the Sundarbans,
Beni-Suef University Journal of Basic and Applied Sciences (2020) 9:61 https://doi.org/10.1186/s43088-020-00081-9
5.
Rahagir
Salekeen, Sadia Noor Mou, Md Emdadul Islam, Asif Ahmed, Md Morsaline Billah, S.M.
Mahbubur Rahman and Kazi Mohammed Didarul Islam 2020. Predicting
Multi-Enzyme Inhibition in the Arachidonic Acid Metabolic Network by Heritiera
fomes Extracts, Journal of Biomolecular Structure & Dynamics, DOI: 10.1080/07391102.2020.1855248
6.
Falguni Guha, Bijoy
Kumar Mondal, S.M. Mahbubur Rahman, Fauzia Begum, Md. Nurul Abser 2020. Antibiotic
Sensitivity for Ready-to-Eat Fish-Based Food-Borne Bacterial Pathogens, J. Pharm. Med. Res. 5(1): 89–92
7.
Mithila
Farjana, Khondoker Moazzem Hossain and Sana Mohammad Mahbubur Rahman
2020. Molecular analysis of the isolated probiotic microorganisms from yoghurt
samples, distributed in Khulna and Chittagong City of Bangladesh, using RAPD
marker, Journal of Bioscience and Agriculture Research, 25(1): 2075-2084.
8.
Tauhidur R. Nurunnabi, Farah Sabrin,
Dilara I. Sharif, Lutfun Nahar, Md. H. Sohrab,
Satyajit D. Sarker, S. M. Mahbubur Rahman
and Md. Morsaline Billah 2020. Antimicrobial activity
of endophytic fungi isolated from the mangrove plant Sonneratia
apetala (Buch. ‑ Ham) from the Sundarbans mangrove forest, Advances
in Traditional Medicine, https://doi.org/10.1007/s13596-019-00422-9
9.
Salauddin Al Azad, Khondoker Moazzem Hossain, Sana
Mohammad
Mahbubur Rahman, Mohammad Faysal Al Mazid, Pallob Barai, Mohammad
Shamim Gazi 2020. In ovo inoculation of duck embryos with different strains of
Bacillus cereus to analyse their synergistic post-hatch antiallergic
potentialities, Veterinary Medicine and Science, 6:992–999. DOI:
10.1002/vms3.279
YEAR
2019
10.
Emdadul
Islam, S.M. Mahbubur Rahman, Md.
Hossain Sohrab, Rana Biswas, Mohammad Shaef Ullah and Kazi Didarul Islam 2019.
Concordance of antioxidant and anti-Inflammatory activity in Xylocarpus
granatum (Koen), J Bangladesh Agril Univ 17(4): 466–475.
11.
Afiya Aunjum,
Rana Biswas, Tauhidur Rahman Nurunnabi, S. M. Mahbubur Rahman,
Md. Morsaline Billah, Md. Emdadul Islam and
Kazi Mohammed Didarul Islam (2019). Antioxidant and antibacterial activity of
three herbs belonging to Zingiber genus of Bangladesh, Oriental Pharmacy and Experimental
Medicine, https://doi.org/10.1007/s13596-019-00403-y
12.
Tauhidur
Rahman Nurunnabi, Shaymaa Al-Majmaie, Lutfun Nahar, Ismini Nakouti, S.M. Mahbubur Rahman, MD. Hossain
Sohrab, MD. Morsaline Billah, Fyaz MD. Ismail, George P. Sharples and Satyajit
D. Sarker 2019. Sonneratinone: A new antimicrobial benzofuranone derivative
from the endophytic fungus Aspergillus niger isolated from the mangrove plant
Sonneratia apetala Buch. - Ham, Trends Phytochem. Res. 3(3): 222-227
13.
Jalil MA, Mahbubur Rahman SM and Hossain KM
2019. Molecular Identification of Lactic Acid Bacteria Isolated from Regional
Yoghurt Samples of Bangladesh Using 16S rDNA Sequencing, Advances in
Pharmacology and Clinical Trials ISSN: 2474-9214, 4(2): 1-9.
14.
Md.
Emdadul Islam, Kazi Mohammed Didarul Islam, Md. Morsaline Billah, Rana Biswas,
Md. Hossain Sohrab and S. M. Mahbubur
Rahman 2019. Antioxidant and anti‑inflammatory
activity of Heritiera fomes (Buch.‑Ham), a mangrove plant of the Sundarbans,
Oriental Pharmacy and Experimental Medicine https://doi.org/10.1007/s13596-019-00401-0
15.
Khandkar
Shaharina Hossain, Sharnali Das, Sushmita Kundu, Shahriar Afrin, Tauhidur
Rahman Nurunnabi and S.M. Mahbubur
Rahman 2019. Isolation and Characterization of Polythene Degrading Bacteria
from Garbage Soil. International Journal of Agriculture, Environment and
Bioresearch Vol. 4, No. 05; ISSN: 2456-8643 www.ijaeb.org Page 254-263.
16.
Rana Biswas, S. M. Mahbubur Rahman, Kazi mohammad
Didarul Islam, Md. morsaline Billah, Afiya Aunjum, Tauhidur Rahman Nurunnabi,
Sujon Kumar kundu and Md. Emdadul Islam 2019. Antioxidant, Anti-inflammatory, and
Anticoagulation Properties of Aegiceras corniculatum and Acanthus ilicifolius, Pharm
Biomed Res 2019; 5(3):35-44.
17.
Sharnali
Das, Tauhidur Rahman Nurunnabi, Rehana Parveen, Amatun Nur Mou, Md. Emdadul
Islam, Kazi Mohammad Didarul Islam and S.M.
Mahbubur Rahman 2019. Isolation and Characterization of Indole Acetic Acid
Producing Bacteria from Rhizosphere Soil and their Effect on Seed Germination. International Journal of Current
Microbiology and Applied Sciences, 8(3):
1237-1245
DOI: https://doi.org/10.20546/ijcmas.2019.803.146
18.
S. M.
Robiul Islam, S. M. Mahbubur Rahman and Md. Shamim Akhter, 2019. Effect
of F. oxysporum extracts on seed
germination and vegetative growth of Abelmoschus
esculentus (Okra). Journa of Bangladesh Army University of Engineering and
Technology, 2(1): 13-20.
YEAR 2018
19.
K.M. Hossain, P. Barai, S.M.M. Rahman,
M.F. Al-Mazid, M.S.Gazi and M.A. Jalil, 2018. Isolation and biochemical
characterization of probiotic bacteria obtained from yogurt samples of Rajshahi
and Chittagong divisions of Bangladesh and their antimicrobial activity against
enteric pathogens, Bangladesh Journal of Livestock Resarch, Special Vol.
(21-25): 142-152.
20.
Pallob Barai, Khondoker Moazzem Hossain, Sana
Mohammad Mahbubur Rahman,
Mohammad Faysal Al Mazid, and Mohammad Shamim Gazi 2018. Antidiarrheal Efficacy
of Probiotic Bacteria in Castor Oil Induced
Diarrheal Mice, Prev. Nutr. Food Sci. 23(4):294-300
https://doi.org/10.3746/pnf.2018.23.4.294;
pISSN 2287-1098ㆍ eISSN
2287-8602
21.
S.
Sultana, S. K. Adhikary, S. M. Mahbubur Rahman M. M. Islam, and 2018. Sexuality
and compatibility of Bipolaris sorokiniana and segregation pattern in telomorph
(Cochliobolus sativa): geographic origin and segregation ratio. Indian Phytopathology,
71(3): 365–375.
22.
Farzana Alam, Kazi M. Didarul Islam and S. M.
Mahbubur Rahman. 2018. Variability among selective guava (Psidium guajava L.) varieties revealed
by morphology and RAPR markers. jahangirnagar University journal of Biological
Sciences. 7(2): 89-98, ISSN 2306-0263.
23.
Tauhidur
Rahman Nurunnabi, Shaymaa Al-Majmaie, Ismini Nakouti, Lutfun Nahar, S.M.
Mahbubur Rahman, Md. Hossain Sohrab, Md. Morsaline Billah, Fyaz m.D.
Ismail, George P. Sharples and Satyajit D. Sarker, 2018. Antimicrobial activity
of kojic acid from the endophytic fungus Colletrichum gloeosporioides isolated
from Sonneratia apetala, a mangrove plant of the Sundarbans, Asian Pacific
Journal of Tropical Medicine, 11(4): 1-5.
24.
S.
Sultana, S. K. Adhikary, M. M. Islam, and S. M. Mahbubur Rahman 2018. Evaluation
of Pathogenic Variability Based on Leaf Blotch Disease Development Components
of Bipolaris sorokiniana in Triticum aestivum and Agroclimatic
Origin, Plant Pathol. J. 34(2) : 93-103
25.
K.M. Hossain,
A. Rahman, S.M.M. Rahman and M.A. Jalil 2018. A study on the anti-allergic
effects of the native probiotic isolates on
β-histine hydrochloride induced mice model, Bang. J. Livs. Res. Special Vol.
21-25, 2018: P. 36-44, ISSN 1022-3851
26.
Tauhidur Rahman Nurunnabi, Lutfun Nahar, Shaymaa
Al-Majmaie, S. M. Mahbubur Rahman,
Md. Hossain sohrab, Md. Morsaline Billah, Fyaz M. D. Ismail, M. Mukhlesur
Rahman, George P. Sharples and Satyajit D. Sarker 2018. Anti-MRSA activity of
oxysporone and xylitol from the endophytic fungus Pestalotia sp. growing on the
Sundarbans mangrove plant Heritiera fomes, Phytotherapy
Research, 32: 348-354.
YEAR
2017
27.
Satyajit D. Sarker, Lutfun Nahar, Tauhidur
Rahman Nurunnabi, S. M. Mahbubur Rahman,
Md. Hossain sohrab, Md. Morsaline Billah, Fyaz M. D. Ismail and George P.
Sharples 2017. A mini-review on oxysporone, Trends
Phytochem Res., 1(2): 55-60.
YEAR 2016
28.
M. Mizanur Rahman, K. Moazzem Hossain and S. M. Mahbubur Rahman (2016).
Isolation, characterization, and properties study of probiotic lactic acid
bacteria of selected yoghurt from Bangladesh. African Journal of Microbiology
Research. Vol. 10(1), pp. 23-31.
YEAR
2015
29.
S. M. Khaledur Rahman, Abdul Jalil, S. M. Mahbubur Rahman and Khondoker
Moazzem Hossain 2015. A study on probiotic properties of isolated and
identified bacteria from regional yoghurts, International
Journal of Bioscience, 7: 139-149.
30.
S. M. Khaledur Rahman, Biplob Kumar dash, Forhad
Karim Shoikat, S. M. Mahbubur Rahman
and Khondoker Moazzem Hossain 2015. Characterization of probiotic bacteria
isolated from regional chicken feces, African
Journal of Microbiology Research, 9: 880-885.
31.
Sifat Tasnim, Rakhi Saha, K. M. Didarul Islam
and S. M. Mahbubur Rahman 2015. PCR
Amplification of 16S rDNA of seven Specis under six Genera of Cucurbitaceae
Family, South Asian J. Agric, 6(1 &
2): 19-21.
YEAR 2012
32.
Md. Shamim Akther, S.M. Mahbubur Rahman and Md. Hafizur Rahman, 2012. Micropropagation
of Bauhina acuminata L., 1(3): 35-43.
33.
M. Mahfuza Khatun, Khondoker Moazzem Hossain and
S.M. Mahbubur Rahman, 2012.
Molecular characterization of Selected Local and Exotic Cattle Using RAPD
Marker, Asian-Aust. J. Anim. Sci., 25(6): 751-757.
YEAR
2010
34.
M.Z Hoque, F. Akter, K.M. Hlssain, M.S.M. Rahman, M.M. Billah and K.M.D.
Islam, 2010. Isolation, Identification and Analysis of Probiotic Properties of
Lactobacillus Spp. from Selective Regional yoghurts, World journal of Dairy
& Food Sciences, 5(1): 39-46.
YEAR
2009
35.
F. Alam, M. A. Mannan and S. M. M. Rahman, 2009. Assessment of Genetic Variability of
Selective Mango (Mangifera indica)
Varieties by RAPD Analysis. South Asian
J. Agric. 4(1&2): 63-66.
36.
F. Akther, S.M.A. Awal and S.M.M. Rahman (2009). Effect of growth
regulators on in vitro multiplication of Dianthus (Dianthus chinensis). South
Asian J. of Agric. 4(1 & 2): 128-131.
YEAR 2008
37.
U. Salma, M.
S. M. Rahman, S. Islam, N. Haque, M. Khatun, T. A. Jubair and B. C. Paul,
2008. Mass Propagation of Rauwolfia serpentina L. Benth, Pakistan journal of Biological Science, 11(9): 1273-1277.
YEAR
2006
38.
S.M.
Mahbubur Rahman, Farhana Afroz, Kaberi Sultana, Palash kumar sen and Md.
Raihan Ali, 2006. Effect of Growth Regulators and State of Media on
Micropropagation of Adhatoda vasica
(Nees), University Studies, Special Issue (1st Research Cell
Conference): 55-59.
39.
M Belal Hossain Sikder, Palash kumar Sen, M
Abdullah-Al Mamun, M. Raihan Ali and S.
M. Mahbubur Rahman, 2006. In Vitro Regeneration of Aromatic Rice (Oryza
sativa L.), International Journal of
Agriculture & Biology, 8(6): 759-762.
40.
Shefali Boonerjee, Palash Kumar Sen, Md. Firoz
Alam and S.M. Mahbubur Rahman, 2006.
In Vitro Clonal Propagation of Onion (Allium cepa L.) through Shoot Tip
Culture, Khulna University Studies,
7(1): 49-53.
41.
Shefali
Boonerjee, Palash Kumar Sen, Md. Firoz Alam and S. M. Mahbubur Rahman 2006.
Micropropagation of Onion (Allium cepa L.), Khulna University Studies, 7(1):
55-58.
YEAR 2005
42.
Md.
Anwar Hossain, Md. Taslim Hossain, Md. Raihan Ali and S. M. Mahbubur Rahman 2005. Effect of Different Carbon Sources on in
vitro Regeneration of Indian Pennywort (Centella asiatica L.), Pakistan
journal of Biological Science, 8(7): 963-965
43.
S. M.
Mahbubur Rahman, Md. Shahidul
Islam, Palash Kumar Sen and Ferdousi Begum 2005. In vitro Propagation of
Oncidium taka, Biotehnology 4(3): 225-229.
YEAR 2004
44.
P.K. Sen,
S.M.M. Rahman, M.F. Hasan, M.A.S. Miah, M.H. Rahman and A.S.M. Noman, 2004.
Biochemical Composition and Enzymatic Analysis of Sugarcane Varieties Isd-16
and Isd-28, Khulna University Studies,
4(2): 775-778.
45.
S.M.
Mahbubur Rahman, Palash Kumar Sen, M. Fida Hasan, M.A. Samad miah and M.
Habibur Rahman, 2004. Purification and Characterization of Invertase Enzyme
from Sugarcane, Pakistan Journal of
Biological Sciences, 7(3): 340-345.
YEAR 2002
46.
S.M.M.
Rahman, M.A. Mamun M.Z. Karim and A.B.M.M. Rahman, 2002. In Vitro
Regeneration of Promising Sugarcane Varieties of Bangladesh, Khulna University Studies, 3(1):
465-469.
47.
S.M.M.
Rahman and M. Yoshida, 2002. Regulation of Seed Dormancy in Cherimoya (Annona cherimola Mill.), Khulna University Studies, 4(1):
677-681.
YEAR 2001
48.
S.M.M.
Rahman, M.R. Ali and T. Shimada, 2001. Genetic Variability of Annona Species Studied by Nucleotide
Sequences, Bangladesh J. Genet.
Biotechnol., 2(1 & 2): 91-99.
YEAR 1998
49.
Mahbubur
Rahman S.M., Takehiko Shimada, Toshiya Yamamoto, John Y. Yonemoto and Masao
Yoshida, 1998. Genetic Diversity of Cherimoya Cultivars Revealed by Amplified
Fragment Length Polymorphism (AFLP) Analysis, Breeding Science 48(1): 5-10.
YEAR 1997
50.
Mahbubur
Rahman S.M., Masahiko Yamada and Masao Yoshida 1997. Relationship of Annona Species as Revealed by PCR-RFLP
Analysis, Breeding Science, 47(4):
335-339.
51.
Mahbubur
Rahman S. M., Takehiko Shimada, John Y. Yonemoto and Masao Yoshida 1997.
Classification and Parentage Determination by RAPD Analysis in Cherimoya (Annona
cherimola Mill.), Mem. Grad. School
Sci. & Technol., Kobe Univ., 15-A: 73-84.
Principles of Genetics
Course: BGE-1205: Principles
of Genetics
Credit
Hours: 03 Year: 1st Term: II
Rationale: The course is designed to provide basic concepts in heredity,
inheritance patterns in plants, animals and microbes, nature of genetic
materials and their transmission pattern, sex determinations mechanisms in
various organisms, mutations and implications in living organisms as well
as in society.
Course Objectives:
• To learn and understand basic principles of genetics, laws of
inheritance at the cellular, individual and population level as well as
basic molecular mechanisms by which genes control growth,
development and morphology.
Intended Learning Outcomes (ILOs):
At the end of the course the students will be able to-
• Understand Mendelian Laws of inheritance at the cellular, individual
and population level.
• Understand basic molecular mechanisms by which genes control
growth, development and morphology of an organism.
• Understand gene linkage and genetic mapping in eukaryotes,
prokaryotes and bacteriophages.
• Learn how Mendelian inheritance differ from Non-Mendelian
inheritance.
• Learn mutations of genetic material and their effects at the
molecular/cellular and organism level.
Course Content
Section – A
1. Introduction: Definition, history, scope and branches of genetics.
Importance of genetics in human society. Heredity and its physical
basis, chromosome theory of heredity, genetic materials, Brief
account of the structure and function of gene. The evaluation of
science of genetics leading to the modern Biotechnology and Genetic
Engineering.
2. Variation: Concept, type and significance of variation in biological
world, natural sources of variation, causes of genetic variation in
asexually reproducing organism (Bacteria, fungi,); Sexual
reproductive cycle (Gametogenesis) of yeast, Drosophila and
mammals.
3. Mendelism: History and Mendel’s experiments with pea plants.
Mendelian Principles I & II and relevant terminology, the physical
basis of law of segregation and law of random assortment. Mendelian
heredity in human and animal.
4. Beyond the Mendelian Genetics: Introduction, types of interactions and
discussion of different kinds of allelic and non-allelic interactions of
genes. Modifiers, expressivity, heterosis, transgressive segregation.
Multiple factors, xenia, multiple allelism and cytoplasmic inheritance.
Section – B
5. Crossing Over: Definition, types and importance of crossing over.
Genetical consequences of crossing over. Theories on the mechanisms
of crossing over. Generalized mechanism of crossing over. Factors
affecting recombination frequencies.
6. Linkage and Mapping: Definition, types and importance of linkage.
History and different hypotheses of linkage, detection of linkage.
Inheritance pattern of linked genes. Genetic and cytological mapping of
chromosomes: Concepts, methods of determining cytological and
genetic mapping.
BGE # 39 BGE # 40
7. Sex Chromosomes and Sex Determination: Discovery of sex
chromosomes, different mechanism of sex determination.
8. Chromosomal Aberrations: Types of structural and numerical changes
of chromosomes and mechanisms, consequences of changing
chromosome structure and number.
9. Gene Mutation: Definition, types, causes & effects of gene mutation
and mechanisms. Mutagens and its types.
Recommended References:
1. Islam, A. S (1973). Fundamentals of Genetics, Humaira Islam, Dhaka.
2. Reddi, O. S (1991). Understanding Genetics. Allied publisher Ltd. New
Delhi Calcutta, India.
3. Sinnot, E. W.; Dunahu, L. C. and Debzhansky. (1958). Principle of
Genetic. Magraw Hill Pvt. Ltd.
4. Strickberger, M. W. (1996). Genetics 3rd ed. Prentice Hall Inc. USA.
5. Lewin. B (1994). Gene V. Oxford University Press, USA, International
Student Edition.
6. Suzuki, et al., (1995). An Introduction to Genetic Analysis. |
Principles of Genetics Sessional and field work
Course: BGE-1206: Principles of Genetics Sessional & Field Work Credit Hour: 01 Year: 1st Term: II Rationale: The course is designed to provide fundamental concepts and practical implications of genetics, inheritance patterns and sex determination mechanisms in animals and plants. Course Objectives: • To apply learned genetical principles in plants like maize, pea plants, tomatoes and laboratory animals like insects (Drosophila spp., honey bee), poultry birds, pigeon, fish, and humans. • Solving conceptual and experimental genetic problems. • To construct pedigree chart. • To construct genetic linkage map. Intended Learning Outcomes (ILOs): At the end of the course the students will be able to- • Apply the principles of inheritance as formulated by Mendel. • Apply the principles of extensions to Mendelian inheritance, including multiple allelism, lethal alleles, gene interactions, and sexlinked transmission. • Analyze experimental genetic problem data using learned genetic principles and statistical procedures. • Construct pedigree chart, genetic and linkage maps. • Genetic counseling in the families and societies to eradicate wrong concepts like sole responsibility of the mother for giving birth of female children only, and some genetic diseases like thalassemia, Down’s syndrome, albinism and consanguineous marriage etc. Course Content 1. Working out the problems on Mendelian inheritance pattern. 2. Working out the problems on modified Mendelian inheritance ratios. 3. Working out the problems on two and three point testcross for linkage and crossing over. 4. Working out the problems on linked genes and blood group inheritance. 5. Study of human genetic traits: PTC taste test and survey of human phenotypic characteristics like- holandric gene effect, fingerprint, iris color, colorblindness, albinism and thalassemia. 6. Study of pea and corn genetics: Dominant-recessive characters, observing recombination and segregation pattern of traits after crossing different corn types. Recommended References: 1. Islam, A. S (1973). Fundamentals of Genetics, Humaira Islam, Dhaka. 2. Reddi, O. S (1991). Understanding Genetics. Allied publisher Ltd. New Delhi Calcutta, India. 3. Sinnot, E. W.; Dunahu, L. C. and Debzhansky. (1958). Principle of Genetic. Magraw Hill Pvt. Ltd. 4. Strickberger, M. W. (1996). Genetics 3rd ed. Prentice Hall Inc. USA. 5. Lewin. B (1994). Gene V. Oxford University Press, USA, International Student Edition. 6. Suzuki, et al., (1995). An Introduction to Genetic Analysis. Running |
Microbial Genetics Section B
Genetics
Credit
Hours: 03 Year: 2nd Term: II
Rationale: This course is designed to introduce different aspects of
microbial genetics. It starts with basic mechanisms of microbial and phage
genetics which extends to their particular applications.
Course Objectives:
• To familiarize with fundamental organization and processes of the
microbial genome, plasmids and gene transfer.
• To introduce phage genetics and applied microbial genetics in
practice.
Intended Learning Outcomes (ILOs):
At the end of the course the students will be able to-
• Learn about the microbial genome, plasmids, and gene transfer
processes e.g. conjugation, transduction and transformation.
• Familiarize with phage genetics, transposable elements and use of
molecular cloning.
Course Content
Section-A
1. Bacterial Chromosome: Introduction, Structure and Replication.
2. Plasmid: Introduction, structure and replication, replication of Col E1,
R6k and conjugative plasmid, control of plasmid replication, plasmid
curing, R-plasmid and antibiotic resistance, mechanism of antibiotic
resistance.
3. Conjugation: F+
and Flike plasmids, tra-operon, sex pili, formation of
hfr strain, gene mapping by conjugation analysis, transfer of nonconjugative plasmid by conjugative plasmid, plasmid mobilization,
and chromosome transfer by cultures of E. coli K-12. Conjugation and
chromosome transfer in other bacteria, conjugal transfer of R-plasmid.
4. Transformation and Electroporation: Competence, uptake of DNA,
transfection. Artificially induced competence. DNA transfer by
electroporation.
5. Transduction: Generalized transduction, experimental evidence, origin
of generalized transducing phages, genetic mapping by different
transductant classes. Specialized transduction, experimental evidence,
origin of specialized transducing phage particle.
Section-B
6. Phage genetics: Phage T4 life cycle (lytic vs. lysogenic), genetic
recombination in T4 phage, genetic fine structure, unit of function.
Phage X 174- life cycle, genetic organization.
BGE # 85 BGE # 86
7. Transposon and Insertion Sequences: Transposable elements, the
mechanism of transposition. Transposon mutagenesis.
8. Molecular Cloning: Restriction endonuclease and digestion of DNA.
Ligases; blunt ended ligation, linker. Homopolymer tailing. Cloning
Vector; use of bacterial plasmid, λ DNA, Cosmid and yeast plasmid as
cloning vector. Gene libraries.
9. Yeast Genetics: Mating type genetics of yeast, yeast plasmid,
mitochondrial inheritance in yeast.
Recommended References:
1. Avers, C. J. (1990). Genetics. Freeman and co. NY.
2. Broce, T. D. Madigan, M. T. Martinco, J. M. and Parker, J. (1990).
Biology of Microorganism.
3. Hardy, K. M. (1986). Bacterial Plasmid. published by American Society
of Microbiology.
4. Strickberger, M. W. (1990). Genetics. Macmillan pub. Co. NY.
5. Suzuki, Griffith and Miller. (1986). Introduction to Genetic Analysis.
W. H. freeman and Co. USA.
6. Gardner, Simmons and Snustad (1991). Principles of Genetics. John
Wiley & Sons.
7. Tortora, Funke and Case (1998). Microbiology- An introduction, Wiley. |
Microbial Genetics Sessional and Field Work
Course: BGE-2212:
Microbial Genetics Sessional
Credit
Hour: 01 Year: 2nd Term: II
Rationale: This course is designed to learn practical skills related to
microbial genetics. It covers laboratory strategies for gene transfer.
Course Objectives:
• To work with bacterial plasmids e.g. isolation and curing.
• To learn gene transfer processes e.g. transformation and conjugation.
Intended Learning Outcomes (ILOs):
At the end of the course the students will be able to-
• Isolate bacterial plasmids, purify them and plasmid curing.
• Conjugate bacteria and transformation of bacterial genetic materials.
Course Content
1. Conjugal transfer of R-Plasmid.
2. Plasmid isolation and curing.
3. Transformation of E. coli K-12 with plasmid DNA.
4. Transduction experiment with available temperate phage and lysogenic
bacteria.
Recommended References:
1. Avers, C. J. (1990). Genetics. Freeman and co. NY.
2. Broce, T. D. Madigan, M. T. Martinco, J. M. and Parker, J. (1990).
Biology of Microorganism.
3. Hardy, K. M. (1986). Bacterial Plasmid. published by American Society
of Microbiology.
4. Strickberger, M. W. (1990). Genetics. Macmillan pub. Co. NY.
5. Suzuki, Griffith and Miller. (1986). Introduction to Genetic Analysis.
W. H. freeman and Co. USA.
6. Gardner, Simmons and Snustad (1991). Principles of Genetics. John
Wiley & Sons.
7. Tortora, Funke and Case (1998). Microbiology- An introduction, Wiley. |
Molecular Bioscience
Section B Running |
Molecular Genetics
A course of 3rd Year Term I |
Techniques in Molecular Biology
A Sessional Course |
- Principles of Genetics
- Principles of Genetics Sessional and field work
- Microbial Genetics Section B
- Microbial Genetics Sessional and Field Work
- Molecular Bioscience
- Molecular Genetics
- Techniques in Molecular Biology