Bacterial nitrogen fixation, also known as biological nitrogen fixation is the alteration of atmospheric nitrogen to ammonia by microorganisms in root nodules of legume plants. This plays a significant aspect in the comprehensive nitrogen cycle and in global agriculture. The introduction of nodulating bacteria into the soil is a good practice for crops because it enhances nutrient and water uptake and minimizes both biotic and abiotic stress. The application of nodulating bacteria for crop cultivation is one of the promising methods for administering nitrogen fertilizers. These bacteria might help in the reduction of external input of fertilizers, greater use of nitrogen by the plant as well as reduced nitrogen loss. Among the leguminous plants, about 95% are associated with bacteria that is they build a symbiotic relationship. These bacteria are symbiotic nitrogen-fixing and produce nodules in the roots, stems and leaves of plants. The minor relationship between leguminous plants and free-living bacteria (endophytes) is reported by many researchers. There are divergent groups of symbiotic bacteria available on the roots of legume plants. However, the identification of these closely related bacterial species is a challenging aspect. Molecular identification using the 16s rRNA gene is a robust technique and common practice for bacteria. Although these free-living endophytes fix nitrogen, they and incapable of inducing root nodules in leguminous plants. This research focuses on the isolation, identification and assessment of symbiotic performances through the observation of growth and yield parameters. Therefore, this research proposal aims to isolate, identify and application of bacterial inoculum to check the growth and yield performance of mung beans.

Bacterial nitrogen fixation, also known as biological nitrogen fixation is the alteration of atmospheric nitrogen to ammonia by microorganisms in root nodules of legume plants. This plays a significant aspect in the comprehensive nitrogen cycle and in global agriculture. The introduction of nodulating bacteria into the soil is a good practice for crops because it enhances nutrient and water uptake and minimizes both biotic and abiotic stress. The application of nodulating bacteria for crop cultivation is one of the promising methods for administering nitrogen fertilizers. These bacteria might help in the reduction of external input of fertilizers, greater use of nitrogen by the plant as well as reduced nitrogen loss. Among the leguminous plants, about 95% are associated with bacteria that is they build a symbiotic relationship. These bacteria are symbiotic nitrogen-fixing and produce nodules in the roots, stems and leaves of plants. The minor relationship between leguminous plants and free-living bacteria (endophytes) is reported by many researchers. There are divergent groups of symbiotic bacteria available on the roots of legume plants. However, the identification of these closely related bacterial species is a challenging aspect. Molecular identification using the 16s rRNA gene is a robust technique and common practice for bacteria. Although these free-living endophytes fix nitrogen, they and incapable of inducing root nodules in leguminous plants. This research focuses on the isolation, identification and assessment of symbiotic performances through the observation of growth and yield parameters. Therefore, this research proposal aims to isolate, identify and application of bacterial inoculum to check the growth and yield performance of mung beans.

• Research Project